Composite

Part:BBa_M50039:Design

Designed by: Anika Naidu   Group: Stanford BIOE44 - S11   (2016-11-30)


E. coli positive feedback cusR producer (short promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 53
    Illegal AgeI site found at 243
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed a genetic construct where the first half consisted of the cusR inducible promoter, a strong ribosome binding site (RBS) listed in the Parts Registry (BBa_B0035) and thecusR gene sourced from NCBI.
BBa M50039 design.png


Source

http://biocyc.org/gene?orgid=ECOLI&id=G6319

References

“cusR response regulator in two-component regulatory system with cusS [Escherichia coli str. K-12 substr. MG1655”. (2016, Sep. 10). NCBI Reference Sequences.

Zahid N, Zulfiqar S, Shakoori AR. (2012, March). Functional analysis of cus operon promoter of Klebsiella pneumoniae using E. coli lacZ assay. Gene, 495(1):81-88.

Loftin IR, et al. (2005, July). A novel copper-binding fold for the periplasmic copper resistance protein cusF. Biochemistry, 44(31):10533-10540.